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random hexanucleotide primer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher random hexanucleotide primer
    Random Hexanucleotide Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+primer+hexanucleotides/random+hexanucleotide+primer/pmc10928104__41419_2024_6576_MOESM3_ESM-90-12-18
    Average 90 stars, based on 1 article reviews
    random hexanucleotide primer - by Bioz Stars, 2026-10
    90/100 stars

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    Reverse Transcription:

    Article Title: Immortalization and transformation of primary human airway epithelial cells by gene transfer.
    Article Snippet: RT ±PCR of RNA from cultured cells was performed using Tri Reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and purifying RNA according to the manufacturer's recommendation. .. RNA was digested with RQ-1 RNAse-free DNAse in the presence of RNAsin (both from Promega, Madison, WI, USA) and repuri®ed using RNEasy columns (Qiagen, Chatsworth, CA, USA). cDNA was made using Random Primer hexanucleotides and Super Script II reverse transcriptase (both from Life Technologies, Inc., Grand Island, NY, USA). ..

    Article Title: Good manufacturing practice production of human corneal limbus-derived stromal stem cells and in vitro quality screening for therapeutic inhibition of corneal scarring
    Article Snippet: Total RNA was extracted using RNeasy Miniprep kit (Qiagen) with on-column RNase-free DNase kit (Qiagen), following manufacturers’ instructions. .. After quantification by Nanodrop (Thermo Fisher), total RNA (500 ng) was reverse-transcribed with SuperScript III Reverse Transcriptase kit (Thermo Fisher) and random primer hexanucleotides (10 ng/ml, Thermo Fisher). .. Complementary DNA (cDNA) was assayed for gene expression using SYBR Green Real-Time Master Mix (Life Technol, Carlsbad, CA) and specific primer (Additional file : Table S2) in a QuantStudio 3 real-time PCR system (Applied Biosystems, Thermo Fisher).



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